5B7J

Structure model of Sap1-DNA complex

Summary for 5B7J

• Entry DOI: 10.2210/pdb5b7j/pdb
• NMR Information: BMRB: 36001
• Descriptor: Switch-activating protein 1, DNA (5'-D(*CP*AP*AP*AP*AP*CP*AP*AP*TP*AP*TP*T)-3'), DNA (5'-D(*AP*AP*TP*AP*TP*TP*GP*TP*TP*TP*TP*G)-3') (3 entities in total)
• Functional Keywords: protein, dna, interaction, complex, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); More
• Cellular location: Nucleus : P40847
• Total number of polymer chains: 3
• Total formula weight: 20569.08

Authors

Jin, C.,Hu, Y.,Ding, J.,Zhang, Y. (deposition date: 2016-06-07, release date: 2017-02-22, Last modification date: 2024-05-01)

Primary citation

Guan, L.,He, P.,Yang, F.,Zhang, Y.,Hu, Y.,Ding, J.,Hua, Y.,Zhang, Y.,Ye, Q.,Hu, J.,Wang, T.,Jin, C.,Kong, D.
Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe
J. Biol. Chem., 292:6056-6075, 2017

PubMed Abstract: A central step in the initiation of chromosomal DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) at late M and early G phase of the cell cycles. Since 1973, four proteins or protein complexes, including cell division control protein 6 (Cdc6)/Cdc18, minichromosome maintenance protein complex, origin recognition complex (ORC), and Cdt1, are known components of the pre-RC. Previously, we reported that a non-ORC protein binds to the essential element Δ9 of the DNA-replication origin ARS3001. In this study, we identified that the non-ORC protein is Sap1. Like ORC, Sap1 binds to DNA origins during cell growth cycles. But unlike ORC, which binds to asymmetric AT-rich sequences through its nine AT-hook motifs, Sap1 preferentially binds to a DNA sequence of 5'-(A/T) (C/G)(A/T)(G/C)(A/T) -3' ( ≥ 1). We also found that Sap1 and ORC physically interact. We further demonstrated that Sap1 is required for the assembly of the pre-RC because of its essential role in recruiting Cdc18 to DNA origins. Thus, we conclude that Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1.

PubMed: 28223353
DOI: 10.1074/jbc.M116.767806

Experimental method

SOLUTION NMR