5B4S
Crystal Structure of GH80 chitosanase from Mitsuaria chitosanitabida
Summary for 5B4S
| Entry DOI | 10.2210/pdb5b4s/pdb |
| Descriptor | Chitosanase (2 entities in total) |
| Functional Keywords | chitosanase, glycoside hydrolase, chitosan, hydrolase |
| Biological source | Mitsuaria chitosanitabida |
| Total number of polymer chains | 2 |
| Total formula weight | 71371.28 |
| Authors | Kumasaka, T.,Yorinaga, Y.,Yamamoto, M.,Hamada, K.,Kawamukai, M. (deposition date: 2016-04-19, release date: 2017-02-01, Last modification date: 2024-11-13) |
| Primary citation | Yorinaga, Y.,Kumasaka, T.,Yamamoto, M.,Hamada, K.,Kawamukai, M. Crystal structure of a family 80 chitosanase from Mitsuaria chitosanitabida FEBS Lett., 591:540-547, 2017 Cited by PubMed Abstract: Chitosanases belong to glycoside hydrolase families 5, 7, 8, 46, 75 and 80 and hydrolyse glucosamine polymers produced by partial or full deacetylation of chitin. Herein, we determined the crystal structure of chitosanase from the β-proteobacterium, Mitsuaria chitosanitabida, (McChoA) at 1.75 Å resolution; the first structure of a family 80 chitosanase. McChoA is a 34 kDa extracellular protein of 301 amino acids that fold into two (upper and lower) globular domains with an active site cleft between them. Key substrate-binding features are conserved with family 24 lysozymes and family 46 chitosanases. The distance between catalytic residues E41 and E61 (10.8 Å) indicates an inverting type mechanism. Uniquely, three disulphide bridges and the C terminus might contribute to enzyme activity. PubMed: 28084023DOI: 10.1002/1873-3468.12557 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.75 Å) |
Structure validation
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