5B39

KIR3DL1*015 in complex with HLA-B*57:01

Summary for 5B39

• Entry DOI: 10.2210/pdb5b39/pdb
• Related: 3VH8 5B38
• Descriptor: HLA class I histocompatibility antigen, B-57 alpha chain, Beta-2-microglobulin, peptide from Ig kappa chain C region, ... (6 entities in total)
• Functional Keywords: innate immune receptor, kir, natural killer cell, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 78144.43

Authors

Vivian, J.P.,Rossjohn, J. (deposition date: 2016-02-12, release date: 2016-03-30, Last modification date: 2024-11-06)

Primary citation

Saunders, P.M.,Pymm, P.,Pietra, G.,Hughes, V.A.,Hitchen, C.,O'Connor, G.M.,Loiacono, F.,Widjaja, J.,Price, D.A.,Falco, M.,Mingari, M.C.,Moretta, L.,McVicar, D.W.,Rossjohn, J.,Brooks, A.G.,Vivian, J.P.
Killer cell immunoglobulin-like receptor 3DL1 polymorphism defines distinct hierarchies of HLA class I recognition
J.Exp.Med., 213:791-807, 2016

PubMed Abstract: Natural killer (NK) cells play a key role in immunity, but how HLA class I (HLA-I) and killer cell immunoglobulin-like receptor 3DL1 (KIR3DL1) polymorphism impacts disease outcome remains unclear. KIR3DL1 (*001/*005/*015) tetramers were screened for reactivity against a panel of HLA-I molecules. This revealed different and distinct hierarchies of specificity for each KIR3DL1 allotype, with KIR3DL1*005 recognizing the widest array of HLA-I ligands. These differences were further reflected in functional studies using NK clones expressing these specific KIR3DL1 allotypes. Unexpectedly, the Ile/Thr80 dimorphism in the Bw4-motif did not categorically define strong/weak KIR3DL1 recognition. Although the KIR3DL1*001, *005, and *015 polymorphisms are remote from the KIR3DL1-HLA-I interface, the structures of these three KIR3DL1-HLA-I complexes showed that the broader HLA-I specificity of KIR3DL1*005 correlated with an altered KIR3DL1*005 interdomain positioning and increased mobility within its ligand-binding site. Collectively, we provide a generic framework for understanding the impact of KIR3DL1 polymorphism on the recognition of HLA-I allomorphs.

PubMed: 27045007
DOI: 10.1084/jem.20152023

Experimental method

X-RAY DIFFRACTION (2.5 Å)