5B2S

Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM)

Summary for 5B2S

Related5B2R 5B2T
DescriptorGuide RNA, CRISPR-associated endonuclease Cas9, Target DNA, ... (9 entities in total)
Functional Keywordscrispr-cas9, genome engineering, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological sourceStreptococcus pyogenes serotype M1
More
Total number of polymer chains4
Total molecular weight196961.12
Authors
Hirano, S.,Nishimasu, H.,Ishitani, R.,Nureki, O. (deposition date: 2016-02-02, release date: 2016-03-23, Last modification date: 2017-10-04)
Primary citation
Hirano, S.,Nishimasu, H.,Ishitani, R.,Nureki, O.
Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9
Mol.Cell, 61:886-894, 2016
PubMed: 26990991 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2016.02.018
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.2 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.23120 2.7% 6.8% 0.64MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
171916
PDB entries from 2020-12-02