5AZV
Crystal structure of hPPARgamma ligand binding domain complexed with 17-oxoDHA
Summary for 5AZV
| Entry DOI | 10.2210/pdb5azv/pdb |
| Related | 5AZT |
| Descriptor | Peroxisome proliferator-activated receptor gamma, (4~{Z},7~{Z},10~{Z},13~{Z},19~{Z})-17-oxidanylidenedocosa-4,7,10,13,19-pentaenoic acid (3 entities in total) |
| Functional Keywords | nuclear receptor, transcription regulation, ligand binding domain, zinc-finger, dna binding, transcription, obesity, nucleus, receptor, activator, oxidized fatty acid, transcription factor, agonist, dual agonist, covalent, ppre, nuclear, dna binding protein |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 2 |
| Total formula weight | 63588.02 |
| Authors | Egawa, D.,Itoh, T.,Yamamoto, K. (deposition date: 2015-10-23, release date: 2016-07-06, Last modification date: 2024-10-23) |
| Primary citation | Egawa, D.,Itoh, T.,Akiyama, Y.,Saito, T.,Yamamoto, K. 17-OxoDHA Is a PPAR alpha/gamma Dual Covalent Modifier and Agonist Acs Chem.Biol., 2016 Cited by PubMed Abstract: 17-Hydroxy docosahexaenoic acid (17-HDHA) is an oxidized form of docosahexaenoic acid (DHA) and known as a specialized proresolving mediator. We found that a further oxidized product, 17-oxodocosahexaenoic acid (17-oxoDHA), activates peroxisome proliferator-activated receptors γ (PPARγ) and PPARα in transcriptional assays and thus can be classified as an α/γ dual agonist. ESI mass spectroscopy and X-ray crystallographic analysis showed that 17-oxoDHA binds to PPARγ and PPARα covalently, making 17-oxoDHA the first of a novel class of PPAR agonists, the PPARα/γ dual covalent agonist. Furthermore, the covalent binding sites were identified as Cys285 for PPARγ and Cys275 for PPARα. PubMed: 27337155DOI: 10.1021/acschembio.6b00338 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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