5AZJ

Crystal structure of glycerol kinase from Trypanosoma brucei gambiense complexed with 4NP (with disulfide bridge)

5AZJ の概要

• エントリーDOI: 10.2210/pdb5azj/pdb
• 関連するPDBエントリー: 5AZI
• 分子名称: Glycerol kinase, 4-NITROPHENYL PHOSPHATE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: glycerol kinase, phosphatase, 4np, african trypanosomes, transferase
• 由来する生物種: Trypanosoma brucei gambiense; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 229225.02

構造登録者

Balogun, E.O.,Inaoka, D.K.,Shiba, T.,Tokuoka, S.M.,Tokumasu, F.,Sakamoto, K.,Michels, P.A.M.,Harada, S.,Kita, K. (登録日: 2015-10-08, 公開日: 2016-10-19, 最終更新日: 2024-10-23)

主引用文献

Balogun, E.O.,Inaoka, D.K.,Shiba, T.,Tokuoka, S.M.,Tokumasu, F.,Sakamoto, K.,Kido, Y.,Michels, P.A.M.,Watanabe, Y.I.,Harada, S.,Kita, K.
Glycerol kinase of African trypanosomes possesses an intrinsic phosphatase activity.
Biochim Biophys Acta Gen Subj, 1861:2830-2842, 2017

PubMed Abstract: In general, glycerol kinases (GKs) are transferases that catalyze phospho group transfer from ATP to glycerol, and the mechanism was suggested to be random bi-bi. The reverse reaction i.e. phospho transfer from glycerol 3-phosphate (G3P) to ADP is only physiologically feasible by the African trypanosome GK. In contrast to other GKs the mechanism of Trypanosoma brucei gambiense glycerol kinase (TbgGK) was shown to be in an ordered fashion, and proceeding via autophosphorylation. From the unique reaction mechanism of TbgGK, we envisaged its potential to possess phosphatase activity in addition to being a kinase.

PubMed: 28778484
DOI: 10.1016/j.bbagen.2017.07.028

実験手法

X-RAY DIFFRACTION (2.61 Å)