5AOH

Crystal Structure of CarF

Summary for 5AOH

• Entry DOI: 10.2210/pdb5aoh/pdb
• Descriptor: Spore coat protein CotH, POTASSIUM ION (3 entities in total)
• Functional Keywords: unknown function, carbapenem resistance, antibiotic resistance, fge homologue
• Biological source: Serratia sp. (strain ATCC 39006)
• Total number of polymer chains: 2
• Total formula weight: 59966.29

Authors

Tichy, E.M.,Hardwick, S.W.,Luisi, B.F.,C Salmond, G.P. (deposition date: 2015-09-10, release date: 2017-01-25, Last modification date: 2024-10-23)

Primary citation

Tichy, E.M.,Hardwick, S.W.,Luisi, B.F.,Salmond, G.P.C.
1.8 angstrom resolution crystal structure of the carbapenem intrinsic resistance protein CarF.
Acta Crystallogr D Struct Biol, 73:549-556, 2017

PubMed Abstract: The natural production of the β-lactam antibiotic carbapenem in bacteria involves a group of enzymes that form a synthetic pathway as well as proteins that protect the cell from self-intoxification by the products. Here, the crystal structure of CarF, one of the two proteins that confer resistance to synthesis of the antibiotic in the host organism, is reported. The CarF fold places it within a widely occurring structural family, indicating an ancient structural origin from which the resistance function has been derived.

PubMed: 28695855
DOI: 10.1107/S2059798317002236

Experimental method

X-RAY DIFFRACTION (1.8 Å)