5AKO

The complex of Tse2 and Tsi2 from Pseudomonas aeruginosa

5AKO の概要

• エントリーDOI: 10.2210/pdb5ako/pdb
• 分子名称: TSI2, TSE2 (3 entities in total)
• 機能のキーワード: unknown function, t6ss, immunity protein, toxin
• 由来する生物種: PSEUDOMONAS AERUGINOSA; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 57015.92

構造登録者

Robb, C.S.,Nano, F.E.,Boraston, A.B. (登録日: 2015-03-04, 公開日: 2015-12-23, 最終更新日: 2024-01-10)

主引用文献

Robb, C.S.,Robb, M.,Nano, F.E.,Boraston, A.B.
The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in Complex with its Immunity Protein.
Structure, 24:277-, 2016

PubMed Abstract: Tse2 is a cytoactive toxin secreted by a type six secretion apparatus of Pseudomonas aeruginosa. The Tse2 toxin naturally attacks a target in the cytoplasm of bacterial cells, and can cause toxicity if artificially introduced into eukaryotic cells. The X-ray crystal structure of the complex of Tse2 and its cognate immunity protein Tsi2 revealed a heterotetrameric structure with an extensive binding interface. Structural identity was found between Tse2 and NAD-dependent enzymes, especially ADP-ribosylating toxins, which facilitated the identification of the Tse2 active site and revealed it to be occluded upon binding the inhibitor Tsi2. The structural identity shared with NAD-dependent enzymes, including conserved catalytic residues, suggests that the mechanism of Tse2 toxicity may be NAD dependent.

PubMed: 26749446
DOI: 10.1016/J.STR.2015.11.012

実験手法

X-RAY DIFFRACTION (2.4 Å)