5AKF

THE CRYSTAL STRUCTURE OF I-DMOI Q42AK120M IN COMPLEX WITH ITS TARGET DNA NICKED IN THE CODING STRAND A AND IN THE PRESENCE OF 2MM MN

Summary for 5AKF

Related5AK9 5AKM 5AKN
DescriptorHOMING ENDONUCLEASE I-DMOI, 5'-D(*GP*CP*CP*TP*TP*GP*CP*CP*GP*GP*GP*TP*AP*AP)-3', 5'-D(*GP*TP*TP*CP*CP*GP*GP*CP*GP*CP*GP)-3', ... (8 entities in total)
Functional Keywordshydrolase, gene targeting, genetics, protein-dna interaction, homing endonucleases
Biological sourceDESULFUROCOCCUS MOBILIS
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Total number of polymer chains12
Total molecular weight115949.44
Authors
Molina, R.,Marcaida, M.J.,Redondo, P.,Marenchino, M.,D'Abramo, M.,Montoya, G.,Prieto, J. (deposition date: 2015-03-03, release date: 2015-06-17, Last modification date: 2017-08-23)
Primary citation
Molina, R.,Marcaida, M.J.,Redondo, P.,Marenchino, M.,Duchateau, P.,D'Abramo, M.,Montoya, G.,Prieto, J.
Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.
J.Biol.Chem., 290:18534-, 2015
PubMed: 26045557 (PDB entries with the same primary citation)
DOI: 10.1074/JBC.M115.658666
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.45 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.1701002.5%0.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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