5AJ9

G7 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa

Summary for 5AJ9

Entry DOI	10.2210/pdb5aj9/pdb
Descriptor	ARYLSULFATASE, CALCIUM ION, SULFATE ION, ... (6 entities in total)
Functional Keywords	hydrolase, catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily
Biological source	PSEUDOMONAS AERUGINOSA
Total number of polymer chains	2
Total formula weight	121792.53
Authors	Miton, C.M.,Fischer, G.,Jonas, S.,Mohammed, M.F.,Loo, B.v.,Kintses, B.,Hyvonen, M.,Tokuriki, N.,Hollfelder, F. (deposition date: 2015-02-20, release date: 2016-03-16, Last modification date: 2019-04-24)
Primary citation	Miton, C.M.,Jonas, S.,Fischer, G.,Duarte, F.,Mohamed, M.F.,van Loo, B.,Kintses, B.,Kamerlin, S.C.L.,Tokuriki, N.,Hyvonen, M.,Hollfelder, F. Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset. Proc. Natl. Acad. Sci. U.S.A., 115:E7293-E7302, 2018 Cited by PubMed: 30012610 DOI: 10.1073/pnas.1607817115 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation