5AA5
Actinobacterial-type NiFe-hydrogenase from Ralstonia eutropha H16 at 2.85 Angstrom resolution
Summary for 5AA5
| Entry DOI | 10.2210/pdb5aa5/pdb |
| Descriptor | NIFE-HYDROGENASE SMALL SUBUNIT, HOFK, NIFE-HYDROGENASE LARGE SUBUNIT, HOFG, IRON/SULFUR CLUSTER, ... (5 entities in total) |
| Functional Keywords | oxidoreductase, hydrogenase, dihydrogen, metalloenzyme, nickel, tropospheric hydrogen, oxygen tolerance |
| Biological source | CUPRIAVIDUS NECATOR (RALSTONIA EUTROPHA) More |
| Total number of polymer chains | 12 |
| Total formula weight | 628610.33 |
| Authors | Schaefer, C.,Bommer, M.,Hennig, S.,Jeoung, J.H.,Dobbek, H.,Lenz, O. (deposition date: 2015-07-23, release date: 2016-01-20, Last modification date: 2024-01-10) |
| Primary citation | Schafer, C.,Bommer, M.,Hennig, S.E.,Jeoung, J.,Dobbek, H.,Lenz, O. Structure of an Actinobacterial-Type [Nife]-Hydrogenase Reveals Insight Into O2-Tolerant H2 Oxidation. Structure, 24:285-, 2016 Cited by PubMed Abstract: A novel group of bacterial [NiFe]-hydrogenases is responsible for high-affinity H2 uptake from the troposphere, and is therefore thought to play an important role in the global H2 cycle. Here we present the first crystal structure at 2.85-Å resolution of such an actinobacterial-type hydrogenase (AH), which was isolated from the dihydrogen oxidizing bacterium, Ralstonia eutropha. The enzyme has a dimeric structure carrying two active [NiFe] sites that are interconnected by six [4Fe4S] clusters over a range of approximately 90 Å. Unlike most other [NiFe]-hydrogenases, the [4Fe4S] cluster proximal to the [NiFe] site is coordinated by three cysteines and one aspartate. Mutagenesis experiments revealed that this aspartate residue is related to the apparent O2 insensitivity of the AH. Our data provide first structural insight into specialized hydrogenases that are supposed to consume atmospheric H2 under challenging conditions, i.e. at high O2 concentration and wide temperature and pH ranges. PubMed: 26749450DOI: 10.1016/J.STR.2015.11.010 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.497 Å) |
Structure validation
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