5A87

Crystal structure of the metallo-beta-lactamase VIM-5

5A87 の概要

• エントリーDOI: 10.2210/pdb5a87/pdb
• 分子名称: METALLO-BETA-LACTAMASE VIM-5, GLYCEROL, ZINC ION, ... (4 entities in total)
• 機能のキーワード: metallo-beta-lactamase, hydrolase, antibiotic resitance
• 由来する生物種: KLEBSIELLA PNEUMONIAE
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52919.39

構造登録者

Brem, J.,Duzgun, A.O.,McDonough, M.A.,Schofield, C.J. (登録日: 2015-07-13, 公開日: 2015-12-23, 最終更新日: 2024-01-10)

主引用文献

Makena, A.,Duzgun, A.O.,Brem, J.,McDonough, M.A.,Rydzik, A.M.,Abboud, M.I.,Saral, A.,Cicek, A.C.,Sandalli, C.,Schofield, C.J.
Comparison of Verona Integron-Borne Metallo-beta-Lactamase (VIM) Variants Reveals Differences in Stability and Inhibition Profiles.
Antimicrob. Agents Chemother., 60:1377-1384, 2015

PubMed Abstract: Metallo-β-lactamases (MBLs) are of increasing clinical significance; the development of clinically useful MBL inhibitors is challenged by the rapid evolution of variant MBLs. The Verona integron-borne metallo-β-lactamase (VIM) enzymes are among the most widely distributed MBLs, with >40 VIM variants having been reported. We report on the crystallographic analysis of VIM-5 and comparison of biochemical and biophysical properties of VIM-1, VIM-2, VIM-4, VIM-5, and VIM-38. Recombinant VIM variants were produced and purified, and their secondary structure and thermal stabilities were investigated by circular dichroism analyses. Steady-state kinetic analyses with a representative panel of β-lactam substrates were carried out to compare the catalytic efficiencies of the VIM variants. Furthermore, a set of metalloenzyme inhibitors were screened to compare their effects on the different VIM variants. The results reveal only small variations in the kinetic parameters of the VIM variants but substantial differences in their thermal stabilities and inhibition profiles. Overall, these results support the proposal that protein stability may be a factor in MBL evolution and highlight the importance of screening MBL variants during inhibitor development programs.

PubMed: 26666919
DOI: 10.1128/AAC.01768-15

実験手法

X-RAY DIFFRACTION (1.5 Å)