5XBM

Structure of SCARB2-JL2 complex

Summary for 5XBM

• Entry DOI: 10.2210/pdb5xbm/pdb
• Descriptor: light chain of JL2, heavy chain of JL2, Lysosome membrane protein 2, ... (7 entities in total)
• Functional Keywords: scarb2, monoclonal antibody, immune system-protein binding complex, immune system/protein binding
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 6
• Total formula weight: 193474.79

Authors

Zhang, X.,Yang, P.,Wang, N.,Zhang, J.,Li, J.,Guo, H.,Yin, X.,Rao, Z.,Wang, X.,Zhang, L. (deposition date: 2017-03-20, release date: 2018-06-27, Last modification date: 2024-10-16)

Primary citation

Zhang, X.,Yang, P.,Wang, N.,Zhang, J.,Li, J.,Guo, H.,Yin, X.,Rao, Z.,Wang, X.,Zhang, L.
The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection.
Protein Cell, 8:590-600, 2017

PubMed Abstract: Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.

PubMed: 28447294
DOI: 10.1007/s13238-017-0405-7

Experimental method

X-RAY DIFFRACTION (3.501 Å)