5XBM
Structure of SCARB2-JL2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 80 |
Detector technology | AREA DETECTOR |
Collection date | 2016-12-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 199.863, 75.646, 164.305 |
Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
Resolution | 49.554 - 3.501 |
R-factor | 0.2409 |
Rwork | 0.238 |
R-free | 0.29310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tw2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.896 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 30522 | |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.7 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 0.2 M Sodium citrate tribasic dehydrate pH 8.3, 20% w/v Polyethylene glycol 3350 |