5VZL
cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex
Summary for 5VZL
Entry DOI | 10.2210/pdb5vzl/pdb |
EMDB information | 8749 |
Descriptor | single guide RNA (116-MER), CRISPR-associated endonuclease Cas9, phage anti-CRISPR AcrIIA4 (3 entities in total) |
Functional Keywords | anti-crispr, cas9, crispr, gene editing, on-target, off-target, cryo-em, immune system-rna complex, immune system/rna |
Biological source | Streptococcus pyogenes M1 GAS More |
Total number of polymer chains | 3 |
Total formula weight | 207247.61 |
Authors | Jiang, F.,Liu, J.J.,Nogales, E.,Doudna, J.A. (deposition date: 2017-05-29, release date: 2017-07-26, Last modification date: 2024-03-13) |
Primary citation | Shin, J.,Jiang, F.,Liu, J.J.,Bray, N.L.,Rauch, B.J.,Baik, S.H.,Nogales, E.,Bondy-Denomy, J.,Corn, J.E.,Doudna, J.A. Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv, 3:e1701620-e1701620, 2017 Cited by PubMed: 28706995DOI: 10.1126/sciadv.1701620 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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