5UGY

Influenza hemagglutinin in complex with a neutralizing antibody

Replaces:  3SM5

Summary for 5UGY

• Entry DOI: 10.2210/pdb5ugy/pdb
• Descriptor: Hemagglutinin HA1, Hemagglutinin HA2, CH65 heavy chain, ... (7 entities in total)
• Functional Keywords: influenza hemagglutinin, viral protein-immune system complex, viral protein/immune system
• Biological source: Influenza A virus (A/Solomon Islands/3/2006(H1N1)); More
• Total number of polymer chains: 12
• Total formula weight: 313208.94

Authors

Whittle, J.R.R.,Jenni, S.,Harrison, S.C. (deposition date: 2017-01-10, release date: 2017-01-25, Last modification date: 2024-10-23)

Primary citation

Whittle, J.R.,Zhang, R.,Khurana, S.,King, L.R.,Manischewitz, J.,Golding, H.,Dormitzer, P.R.,Haynes, B.F.,Walter, E.B.,Moody, M.A.,Kepler, T.B.,Liao, H.X.,Harrison, S.C.
Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin.
Proc. Natl. Acad. Sci. U.S.A., 108:14216-14221, 2011

PubMed Abstract: Seasonal antigenic drift of circulating influenza virus leads to a requirement for frequent changes in vaccine composition, because exposure or vaccination elicits human antibodies with limited cross-neutralization of drifted strains. We describe a human monoclonal antibody, CH65, obtained by isolating rearranged heavy- and light-chain genes from sorted single plasma cells, coming from a subject immunized with the 2007 trivalent influenza vaccine. The crystal structure of a complex of the hemagglutinin (HA) from H1N1 strain A/Solomon Islands/3/2006 with the Fab of CH65 shows that the tip of the CH65 heavy-chain complementarity determining region 3 (CDR3) inserts into the receptor binding pocket on HA1, mimicking in many respects the interaction of the physiological receptor, sialic acid. CH65 neutralizes infectivity of 30 out of 36 H1N1 strains tested. The resistant strains have a single-residue insertion near the rim of the sialic-acid pocket. We conclude that broad neutralization of influenza virus can be achieved by antibodies with contacts that mimic those of the receptor.

PubMed: 21825125
DOI: 10.1073/pnas.1111497108

Experimental method

X-RAY DIFFRACTION (2.801 Å)