Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 154.824, 192.190, 333.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.923 - 2.801 |
| R-factor | 0.2598 |
| Rwork | 0.259 |
| R-free | 0.28880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rvz |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.923 | 29.923 | 2.870 |
| High resolution limit [Å] | 2.800 | 12.520 | 2.800 |
| Rmerge | 0.145 | 0.028 | 5.576 |
| Rmeas | 0.159 | 0.031 | 6.409 |
| Total number of observations | 653278 | ||
| Number of reflections | 114569 | 1469 | 6356 |
| <I/σ(I)> | 11.09 | 44.91 | 0.31 |
| Completeness [%] | 94.0 | 96.8 | 70.9 |
| Redundancy | 5.702 | 5.332 | 3.821 |
| CC(1/2) | 0.996 | 0.999 | 0.131 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2.2 M ammonium sulfate, 100 mM Tris, 5% PEG400, pH 7.5 |
