5OYD

GH5 endo-xyloglucanase from Cellvibrio japonicus

Summary for 5OYD

• Entry DOI: 10.2210/pdb5oyd/pdb
• Descriptor: Cellulase, putative, cel5D, beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-N-acetyl-beta-D-glucopyranosylamine, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-N-acetyl-beta-D-glucopyranosylamine, ... (7 entities in total)
• Functional Keywords: endo-xyloglucanase, hydrolase
• Biological source: Cellvibrio japonicus
• Total number of polymer chains: 2
• Total formula weight: 91495.63

Authors

Attia, M.,Nelson, C.E.,Offen, W.A.,Jain, N.,Gardner, J.G.,Davies, G.J.,Brumer, H. (deposition date: 2017-09-08, release date: 2018-02-07, Last modification date: 2024-10-09)

Primary citation

Attia, M.A.,Nelson, C.E.,Offen, W.A.,Jain, N.,Davies, G.J.,Gardner, J.G.,Brumer, H.
In vitro and in vivo characterization of threeCellvibrio japonicusglycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions.
Biotechnol Biofuels, 11:45-45, 2018

PubMed Abstract: Xyloglucan (XyG) is a ubiquitous and fundamental polysaccharide of plant cell walls. Due to its structural complexity, XyG requires a combination of backbone-cleaving and sidechain-debranching enzymes for complete deconstruction into its component monosaccharides. The soil saprophyte has emerged as a genetically tractable model system to study biomass saccharification, in part due to its innate capacity to utilize a wide range of plant polysaccharides for growth. Whereas the downstream debranching enzymes of the xyloglucan utilization system of have been functionally characterized, the requisite backbone-cleaving -xyloglucanases were unresolved.

PubMed: 29467823
DOI: 10.1186/s13068-018-1039-6

Experimental method

X-RAY DIFFRACTION (2.1 Å)