5OIK
Structure of an RNA polymerase II-DSIF transcription elongation complex
Summary for 5OIK
| Entry DOI | 10.2210/pdb5oik/pdb |
| Related | 5oho 5ohq |
| EMDB information | 3815 3816 3817 3818 3819 |
| Descriptor | DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (19 entities in total) |
| Functional Keywords | rna polymerase ii, transcription elongation, transcription |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 17 |
| Total formula weight | 694730.49 |
| Authors | Bernecky, C.,Plitzko, J.M.,Cramer, P. (deposition date: 2017-07-18, release date: 2017-09-13, Last modification date: 2024-05-15) |
| Primary citation | Bernecky, C.,Plitzko, J.M.,Cramer, P. Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp. Nat. Struct. Mol. Biol., 24:809-815, 2017 Cited by PubMed Abstract: During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4 Å. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis. PubMed: 28892040DOI: 10.1038/nsmb.3465 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
Download full validation report
