5OIK

Structure of an RNA polymerase II-DSIF transcription elongation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase II subunit RPB1	polymer	1970	217436.1	1	Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) UniProt (by SIFTS) (G3MZY8) In PDB	Bos taurus
2	B	DNA-directed RNA polymerase subunit beta	polymer	1174	134041.4	1	UniProt (A5PJW8) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB	Bos taurus (Bovine)
3	C	DNA-directed RNA polymerase II subunit RPB3	polymer	275	31466.1	1	UniProt (Q3T0Q3) Pfam (PF01193) Pfam (PF01000) In PDB	Bos taurus (Bovine)	RNA polymerase II subunit B3,DNA-directed RNA polymerase II subunit C
4	D	Polymerase (RNA) II (DNA directed) polypeptide D	polymer	142	16347.3	1	UniProt (Q1JQ91) Pfam (PF03874) In PDB	Bos taurus (Bovine)
5	E	DNA-directed RNA polymerases I, II, and III subunit RPABC1	polymer	210	24514.2	1	UniProt (Q2T9T3) Pfam (PF03871) Pfam (PF01191) In PDB	Bos taurus (Bovine)	RNA polymerases I,II,and III subunit ABC1,DNA-directed RNA polymerase II subunit E,RPB5 homolog
6	F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	127	14491.0	1	UniProt (F2Z4C9) Pfam (PF01192) UniProt (by SIFTS) (Q32PE0) In PDB	Bos taurus (Bovine)
7	G	DNA-directed RNA polymerase II subunit RPB7	polymer	172	19314.3	1	UniProt (Q5E9B8) Pfam (PF03876) Pfam (PF00575) In PDB	Bos taurus (Bovine)	RNA polymerase II subunit B7,DNA-directed RNA polymerase II subunit G
8	H	Uncharacterized protein	polymer	150	17162.3	1	UniProt (F2Z4H3) Pfam (PF03870) In PDB	Bos taurus (Bovine)
9	I	DNA-directed RNA polymerase II subunit RPB9	polymer	125	14507.2	1	UniProt (Q32P73) Pfam (PF02150) Pfam (PF01096) In PDB	Bos taurus (Bovine)	RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I
10	J	DNA-directed RNA polymerases I, II, and III subunit RPABC5	polymer	67	7655.1	1	UniProt (Q32P78) Pfam (PF01194) In PDB	Bos taurus (Bovine)	RNA polymerases I,II,and III subunit ABC5,DNA-directed RNA polymerase III subunit L
11	K	DNA-directed RNA polymerase II subunit RPB11	polymer	117	13310.3	1	UniProt (Q32P79) Pfam (PF13656) In PDB	Bos taurus (Bovine)	RNA polymerase II subunit B11,DNA-directed RNA polymerase II subunit J
12	L	DNA-directed RNA polymerases I, II, and III subunit RPABC4	polymer	58	7018.2	1	UniProt (Q3ZBC0) Pfam (PF03604) In PDB	Bos taurus (Bovine)	RNA polymerases I,II,and III subunit ABC4,DNA-directed RNA polymerase II subunit K
13	N	DNA (43-MER)	polymer	43	13303.6	1		synthetic construct
14	P	RNA (5'-R(P*UP*AP*UP*AP*UP*AP*CP*AP*UP*AP*AP*AP*GP*AP*CP*CP*AP*GP*GP*C)-3')	polymer	50	16081.7	1		synthetic construct
15	T	DNA (43-MER)	polymer	43	13178.5	1		synthetic construct
16	Y	Transcription elongation factor SPT4	polymer	117	13210.2	1	UniProt (P63272) Pfam (PF06093) In PDB	Homo sapiens (Human)	hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit
17	Z	Transcription elongation factor SPT5	polymer	1087	121145.5	1	UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) In PDB	Homo sapiens (Human)	hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein
18	A, B, I, J, L...	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
19	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	17
	Total formula weight	694182.9
Non-Polymers*	Number of molecules	9
	Total formula weight	547.6
All*	Total formula weight	694730.5

*Water molecules are not included.