5E6S

Structures of leukocyte integrin aLB2: The aI domain, the headpiece, and the pocket for the internal ligand

Summary for 5E6S

• Entry DOI: 10.2210/pdb5e6s/pdb
• Related: 5E6R 5E6U
• Descriptor: Integrin alpha-L, Integrin beta-2, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: lymphocyte function-associated antigen-1, cell adhesion
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 6
• Total formula weight: 436887.64

Authors

Springer, T.A.,Sen, M. (deposition date: 2015-10-10, release date: 2016-03-02, Last modification date: 2020-07-29)

Primary citation

Sen, M.,Springer, T.A.
Leukocyte integrin alpha L beta 2 headpiece structures: The alpha I domain, the pocket for the internal ligand, and concerted movements of its loops.
Proc.Natl.Acad.Sci.USA, 113:2940-2945, 2016

PubMed Abstract: High-resolution crystal structures of the headpiece of lymphocyte function-associated antigen-1 (integrin αLβ2) reveal how the αI domain interacts with its platform formed by the α-subunit β-propeller and β-subunit βI domains. The αLβ2 structures compared with αXβ2 structures show that the αI domain, tethered through its N-linker and a disulfide to a stable β-ribbon pillar near the center of the platform, can undergo remarkable pivoting and tilting motions that appear buffered by N-glycan decorations that differ between αL and αX subunits. Rerefined β2 integrin structures reveal details including pyroglutamic acid at the β2 N terminus and bending within the EGF1 domain. Allostery is relayed to the αI domain by an internal ligand that binds to a pocket at the interface between the β-propeller and βI domains. Marked differences between the αL and αX subunit β-propeller domains concentrate near the binding pocket and αI domain interfaces. Remarkably, movement in allostery in the βI domain of specificity determining loop 1 (SDL1) causes concerted movement of SDL2 and thereby tightens the binding pocket for the internal ligand.

PubMed: 26936951
DOI: 10.1073/pnas.1601379113

Experimental method

X-RAY DIFFRACTION (2.15 Å)