5C88

Crystal structure of Ard1 N-terminal acetyltransferase from Sulfolobus solfataricus in monoclinic form

Summary for 5C88

Entry DOI	10.2210/pdb5c88/pdb
Descriptor	Uncharacterized N-acetyltransferase SSO0209, COENZYME A (3 entities in total)
Functional Keywords	acetyltransferase, transferase
Biological source	Sulfolobus solfataricus strain P2
Cellular location	Cytoplasm : Q980R9
Total number of polymer chains	2
Total formula weight	42139.95
Authors	Chang, Y.Y.,Hsu, C.H. (deposition date: 2015-06-25, release date: 2016-01-13, Last modification date: 2023-11-08)
Primary citation	Chang, Y.Y.,Hsu, C.H. Multiple Conformations of the Loop Region Confers Heat-Resistance on SsArd1, a Thermophilic NatA. Chembiochem, 17:214-217, 2016 Cited by PubMed Abstract: Structural comparison indicates that the loop region between β3 and β4 of SsArd1 is extended relative to the corresponding region in mesophilic Nats, and forms a plastic hydrogen-bond network mainly at two serine residues. Strikingly, two single-point mutants showed ∼3 °C decrease in melting temperature, and two other variants showed ∼7 °C decrease; this correlated with significantly reduced enzymatic activity. To our knowledge, this is the first discovery of a loop region capable of remarkably improving protein thermostability. This provides a novel route to engineer heat-resistant proteins. PubMed: 26593285 DOI: 10.1002/cbic.201500568 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.49 Å)

Structure validation