4ZWJ

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Summary for 4ZWJ

DescriptorChimera protein of human Rhodopsin, mouse S-arrestin, and T4 Endolysin (1 entity in total)
Functional Keywordsgpcr, rhodopsin, visual arrestin, x-ray free electron laser, serial femtosecond crystallography, signaling protein, structural genomics, gpcr network
Biological sourceEnterobacteria phage T4
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Cellular locationCell projection, cilium, photoreceptor outer segment  P20443
Total number of polymer chains4
Total molecular weight403444.41
Authors
Kang, Y.,Zhou, X.E.,Gao, X.,He, Y.,Liu, W.,Ishchenko, A.,Barty, A.,White, T.A.,Yefanov, O.,Han, G.W.,Xu, Q.,de Waal, P.W.,Ke, J.,Tan, M.H.E.,Zhang, C.,Moeller, A.,West, G.M.,Pascal, B.,Eps, N.V.,Caro, L.N.,Vishnivetskiy, S.A.,Lee, R.J.,Suino-Powell, K.M.,Gu, X.,Pal, K.,Ma, J.,Zhi, X.,Boutet, S.,Williams, G.J.,Messerschmidt, M.,Gati, C.,Zatsepin, N.A.,Wang, D.,James, D.,Basu, S.,Roy-Chowdhury, S.,Conrad, C.,Coe, J.,Liu, H.,Lisova, S.,Kupitz, C.,Grotjohann, I.,Fromme, R.,Jiang, Y.,Tan, M.,Yang, H.,Li, J.,Wang, M.,Zheng, Z.,Li, D.,Howe, N.,Zhao, Y.,Standfuss, J.,Diederichs, K.,Dong, Y.,Potter, C.S.,Carragher, B.,Caffrey, M.,Jiang, H.,Chapman, H.N.,Spence, J.C.H.,Fromme, P.,Weierstall, U.,Ernst, O.P.,Katritch, V.,Gurevich, V.V.,Griffin, P.R.,Hubbell, W.L.,Stevens, R.C.,Cherezov, V.,Melcher, K.,Xu, H.E.,GPCR Network (GPCR) (deposition date: 2015-05-19, release date: 2015-07-29, Last modification date: 2018-02-14)
Primary citation
Kang, Y.,Zhou, X.E.,Gao, X.,He, Y.,Liu, W.,Ishchenko, A.,Barty, A.,White, T.A.,Yefanov, O.,Han, G.W.,Xu, Q.,de Waal, P.W.,Ke, J.,Tan, M.H.,Zhang, C.,Moeller, A.,West, G.M.,Pascal, B.D.,Van Eps, N.,Caro, L.N.,Vishnivetskiy, S.A.,Lee, R.J.,Suino-Powell, K.M.,Gu, X.,Pal, K.,Ma, J.,Zhi, X.,Boutet, S.,Williams, G.J.,Messerschmidt, M.,Gati, C.,Zatsepin, N.A.,Wang, D.,James, D.,Basu, S.,Roy-Chowdhury, S.,Conrad, C.E.,Coe, J.,Liu, H.,Lisova, S.,Kupitz, C.,Grotjohann, I.,Fromme, R.,Jiang, Y.,Tan, M.,Yang, H.,Li, J.,Wang, M.,Zheng, Z.,Li, D.,Howe, N.,Zhao, Y.,Standfuss, J.,Diederichs, K.,Dong, Y.,Potter, C.S.,Carragher, B.,Caffrey, M.,Jiang, H.,Chapman, H.N.,Spence, J.C.,Fromme, P.,Weierstall, U.,Ernst, O.P.,Katritch, V.,Gurevich, V.V.,Griffin, P.R.,Hubbell, W.L.,Stevens, R.C.,Cherezov, V.,Melcher, K.,Xu, H.E.
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.
Nature, 523:561-567, 2015
PubMed: 26200343 (PDB entries with the same primary citation)
DOI: 10.1038/nature14656
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.302 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.292100.6%4.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution