4ZT0

Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution

Summary for 4ZT0

Related4ZT9
DescriptorCRISPR-associated endonuclease Cas9, single-guide RNA (2 entities in total)
Functional Keywordscrispr-cas9, bacteria adaptive immunity, dna endonuclease, hydrolase-rna complex, genome editing and regulation, hydrolase/rna
Biological sourceStreptococcus pyogenes
Total number of polymer chains4
Total molecular weight374612
Authors
Jiang, F.,Doudna, J.A. (deposition date: 2015-05-14, release date: 2015-07-08)
Primary citation
Jiang, F.,Zhou, K.,Ma, L.,Gressel, S.,Doudna, J.A.
A Cas9-guide RNA complex preorganized for target DNA recognition.
Science, 348:1477-1481, 2015
PubMed: 26113724 (PDB entries with the same primary citation)
DOI: 10.1126/science.aab1452
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.9 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.26840.4%1.3%4.7%0.53MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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More Biological unit images

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Molmil generated image of 4zt0
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (4zt0.pdb1.gz [528.25 KB])
Coordinate files for Biological unit (4zt0.pdb2.gz [504.9 KB])