4ZMV

Crystal structure of human P-cadherin (ss-X-dimer pocket I)

4ZMV の概要

• エントリーDOI: 10.2210/pdb4zmv/pdb
• 関連するPDBエントリー: 4zml 4zmn 4zmo 4zmp 4zmq 4zmt 4zmw 4zmx 4zmy 4zmz
• 分子名称: Cadherin-3, CALCIUM ION, NICKEL (II) ION, ... (6 entities in total)
• 機能のキーワード: dimerization, conformational change, cell adhesion
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47799.03

構造登録者

Caaveiro, J.M.M.,Kudo, S.,Tsumoto, K. (登録日: 2015-05-04, 公開日: 2016-09-07, 最終更新日: 2023-11-08)

主引用文献

Kudo, S.,Caaveiro, J.M.,Tsumoto, K.
Adhesive Dimerization of Human P-Cadherin Catalyzed by a Chaperone-like Mechanism
Structure, 24:1523-1536, 2016

PubMed Abstract: Orderly assembly of classical cadherins governs cell adhesion and tissue maintenance. A key event is the strand-swap dimerization of the extracellular ectodomains of two cadherin molecules from apposing cells. Here we have determined crystal structures of P-cadherin in six different conformational states to elaborate a motion picture of its adhesive dimerization at the atomic level. The snapshots revealed that cell-adhesive dimerization is facilitated by several intermediate states collectively termed X-dimer in analogy to other classical cadherins. Based on previous studies and on the combined structural, kinetic, thermodynamic, biochemical, and cellular data reported herein, we propose that the adhesive dimerization of human P-cadherin is achieved by a stepwise mechanism analogous to that of assembly chaperones. This mechanism, applicable to type I classical cadherins, confers high specificity and fast association rates. We expect these findings to guide innovative therapeutic approaches targeting P-cadherin in cancer.

PubMed: 27545624
DOI: 10.1016/j.str.2016.07.002

実験手法

X-RAY DIFFRACTION (2.4 Å)