4ZMV
Crystal structure of human P-cadherin (ss-X-dimer pocket I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.440, 98.910, 109.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.100 - 2.400 |
| R-factor | 0.20769 |
| Rwork | 0.206 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zmn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.664 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.605 |
| Number of reflections | 33412 | |
| <I/σ(I)> | 11.1 | 2.6 |
| Completeness [%] | 98.2 | 97.7 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2 M Lithium sulfate 10 mM nickel sulfate 100 mM TRIS |
