4ZLT

Crystal structure of viral chemokine binding protein R17 in complex with CCL3

4ZLT の概要

• エントリーDOI: 10.2210/pdb4zlt/pdb
• 関連するPDBエントリー: 2X6G 4ZKQ
• 分子名称: Putative uncharacterized protein, C-C motif chemokine 3, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: rhvp chemokine binding protein in complex with chemokine ccl3, chemokine binding protein-chemokine complex, chemokine binding protein/chemokine
• 由来する生物種: Cricetid herpesvirus 2; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 111626.21

構造登録者

Lubman, O.Y.,Fremont, D.H. (登録日: 2015-05-01, 公開日: 2015-11-18, 最終更新日: 2024-11-13)

主引用文献

Lubman, O.Y.,Fremont, D.H.
Parallel Evolution of Chemokine Binding by Structurally Related Herpesvirus Decoy Receptors.
Structure, 24:57-69, 2016

PubMed Abstract: A wide variety of pathogens targets chemokine signaling networks in order to disrupt host immune surveillance and defense. Here, we report a structural and mutational analysis of rodent herpesvirus Peru encoded R17, a potent chemokine inhibitor that sequesters CC and C chemokines with high affinity. R17 consists of a pair of β-sandwich domains linked together by a bridging sheet, which form an acidic binding cleft for the chemokine CCL3 on the opposite face of a basic surface cluster that binds glycosaminoglycans. R17 promiscuously engages chemokines primarily through the same N-loop determinants used for host receptor recognition while residues located in the chemokine 40s loop drive kinetically stable complex formation. The core fold adopted by R17 is unexpectedly similar to that of the M3 chemokine decoy receptor encoded by MHV-68, although, strikingly, neither the location of ligand engagement nor the stoichiometry of binding is conserved, suggesting that their functions evolved independently.

PubMed: 26671708
DOI: 10.1016/j.str.2015.10.018

実験手法

X-RAY DIFFRACTION (3 Å)