4ZK7

Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31

4ZK7 の概要

• エントリーDOI: 10.2210/pdb4zk7/pdb
• 分子名称: Chorismate mutase, Divalent-cation tolerance protein CutA (2 entities in total)
• 機能のキーワード: tetrahedral, computational design, rosetta, self-assembly, symmetric, nanomaterial, solubility, protein binding
• 由来する生物種: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579); 詳細
• 細胞内の位置: Cytoplasm : Q5SJY4 Q7SIA8
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 324480.56

構造登録者

Liu, Y.,Cascio, D.,Sawaya, M.R.,Bale, J.,Collazo, M.J.,Park, R.,King, N.,Baker, D.,Yeates, T. (登録日: 2015-04-30, 公開日: 2015-07-29, 最終更新日: 2024-03-06)

主引用文献

Bale, J.B.,Park, R.U.,Liu, Y.,Gonen, S.,Gonen, T.,Cascio, D.,King, N.P.,Yeates, T.O.,Baker, D.
Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.
Protein Sci., 24:1695-1701, 2015

PubMed Abstract: We recently reported the development of a computational method for the design of coassembling multicomponent protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials.

PubMed: 26174163
DOI: 10.1002/pro.2748

実験手法

X-RAY DIFFRACTION (3.4 Å)