4ZK3

Psoriasis pathogenesis - Pso p27 constitute a compact structure forming large aggregates. Low pH structure

4ZK3 の概要

• エントリーDOI: 10.2210/pdb4zk3/pdb
• 関連するPDBエントリー: 4ZK0
• 分子名称: Serpin B3 (2 entities in total)
• 機能のキーワード: immune system, autoimmunity, pathogenesis, pso p27, pso p27-complex, psoriasis, scca1, serpinb3, hydrolase inhibitor
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm : P29508
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44620.45

構造登録者

Helland, R.,Lysvand, H.,Slupphaug, G.,Iversen, O.J. (登録日: 2015-04-29, 公開日: 2015-07-01, 最終更新日: 2024-05-08)

主引用文献

Lysvand, H.,Helland, R.,Hagen, L.,Slupphaug, G.,Iversen, O.J.
Psoriasis pathogenesis - Pso p27 constitutes a compact structure forming large aggregates.
Biochem Biophys Rep, 2:132-136, 2015

PubMed Abstract: Psoriasis is a chronic inflammatory skin disease. The absence of microbial organisms as potential causal agents has given rise to the hypothesis that the inflammation is due to an autoimmune reaction. The defined inflamed areas of the skin lesions argue for an immunological disease with a local production of a causal antigen. Pso p27 is a protein generated in mast cells in psoriatic plaques, but not in uninvolved skin. We recently demonstrated that the Pso p27 is generated by cleavage of SerpinB3 (SCCA1) in the presence of mast cell associated chymase. In this communication we demonstrate by X-ray crystallographic analysis that the cleavage products associate into a complex similar to SCCA1, but with the reactive centre loop inserted into a 5-stranded central β-sheet. Native gel electrophoresis show that these Pso p27 complexes form large aggregates which may be of significance with respect to an immunogenic role of Pso p27.

PubMed: 29124154
DOI: 10.1016/j.bbrep.2015.06.001

実験手法

X-RAY DIFFRACTION (2 Å)