4ZFP

A new crystal structure for the adduct formed in the reaction between AuSac2, a cytotoxic homoleptic gold(I) compound with the saccharinate ligand, and the model protein hen egg white lysozyme

Summary for 4ZFP

• Entry DOI: 10.2210/pdb4zfp/pdb
• Descriptor: Lysozyme C, 1,2-ETHANEDIOL, NITRATE ION, ... (5 entities in total)
• Functional Keywords: gold compounds, gold-based drugs, protein-metallodrug interactions, metallodrugs, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 15356.16

Authors

Merlino, A. (deposition date: 2015-04-21, release date: 2015-06-24, Last modification date: 2024-11-20)

Primary citation

Ferraro, G.,Massai, L.,Messori, L.,Cinellu, M.A.,Merlino, A.
Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme.
Biometals, 28:745-754, 2015

PubMed Abstract: A new crystal structure is reported here for the adduct formed in the reaction between NH4 [Au(Sac)2], AuSac2, a cytotoxic homoleptic gold(I) complex with the saccharinate ligand, and the model protein hen egg white lysozyme. To produce this adduct, AuSac2 breaks down and releases both saccharinate ligands. The resulting Au(I) ions bind the protein to ND1 and NE2 atoms of His15 but also to SD atom of the zero-solvent accessible Met105 side chain, which is located in the protein hydrophobic box. The unexpected existence of this secondary gold(I) binding site is confirmed by spectroscopic and spectrometric measurements in solution.

PubMed: 26054833
DOI: 10.1007/s10534-015-9863-7

Experimental method

X-RAY DIFFRACTION (1.96 Å)