4ZB1
Crystal Structure of Blue Chromoprotein sgBP from Stichodactyla Gigantea
Summary for 4ZB1
| Entry DOI | 10.2210/pdb4zb1/pdb |
| Descriptor | Blue chromoprotein, sgBP, 2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL (3 entities in total) |
| Functional Keywords | blue chromoprotein, luminescent protein |
| Biological source | Stichodactyla gigantea |
| Total number of polymer chains | 2 |
| Total formula weight | 56660.35 |
| Authors | Lee, C.C.,Ching, C.Y.,Tsai, H.J.,Wang, A.H.J. (deposition date: 2015-04-14, release date: 2015-08-12, Last modification date: 2024-10-23) |
| Primary citation | Chiang, C.Y.,Lee, C.C.,Lo, S.Y.,Wang, A.H.,Tsai, H.J. Chromophore Deprotonation State Alters the Optical Properties of Blue Chromoprotein Plos One, 10:e0134108-e0134108, 2015 Cited by PubMed Abstract: Chromoproteins (CPs) have unique colors and can be used in biological applications. In this work, a novel blue CP with a maximum absorption peak (λmax) at 608 nm was identified from the carpet anemone Stichodactyla gigantea (sgBP). In vivo expression of sgBP in zebrafish would change the appearance of the fishes to have a blue color, indicating the potential biomarker function. To enhance the color properties, the crystal structure of sgBP at 2.25 Å resolution was determined to allow structure-based protein engineering. Among the mutations conducted in the Gln-Tyr-Gly chromophore and chromophore environment, a S157C mutation shifted the λmax to 604 nm with an extinction coefficient (ε) of 58,029 M-1·cm-1 and darkened the blue color expression. The S157C mutation in the sgBP chromophore environment could affect the color expression by altering the deprotonation state of the phenolic group in the chromophore. Our results provide a structural basis for the blue color enhancement of the biomarker development. PubMed: 26218063DOI: 10.1371/journal.pone.0134108 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.25 Å) |
Structure validation
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