4Z46

X-ray structure of the bis-platinum lysozyme adduct formed in the reaction between the protein and the two drugs Cisplatin and Oxaliplatin

4Z46 の概要

• エントリーDOI: 10.2210/pdb4z46/pdb
• 分子名称: Lysozyme C, 1,2-ETHANEDIOL, NITRATE ION, ... (7 entities in total)
• 機能のキーワード: cisplatin, oxaliplatin, protein platination, anticancer drugs, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15668.63

構造登録者

Merlino, A. (登録日: 2015-04-01, 公開日: 2015-05-27, 最終更新日: 2024-10-16)

主引用文献

Marasco, D.,Messori, L.,Marzo, T.,Merlino, A.
Oxaliplatin vs. cisplatin: competition experiments on their binding to lysozyme.
Dalton Trans, 44:10392-10398, 2015

PubMed Abstract: The model protein hen egg white lysozyme was challenged with oxaliplatin and cisplatin. ESI mass spectrometry, surface plasmon resonance and thermal shift analyses demonstrate the formation of a bis-platinum adduct, though in very small amounts. Crystals of the bis-platinum adduct were obtained using two different preparations and the X-ray structures were solved at 1.85 Å and 1.95 Å resolution. Overall, the obtained data point out that, under the analyzed conditions, the two Pt drugs have similar affinities for the protein, but bind on its surface at two non-overlapping sites. In other words, these two drugs manifest a significantly different reactivity with this model protein and do not compete for the same protein binding sites.

PubMed: 25974859
DOI: 10.1039/c5dt01279a

実験手法

X-RAY DIFFRACTION (1.85 Å)