4Z44

F454K Mutant of Tryptophan 7-halogenase PrnA

4Z44 の概要

• エントリーDOI: 10.2210/pdb4z44/pdb
• 関連するPDBエントリー: 4Z43
• 分子名称: Flavin-dependent tryptophan halogenase PrnA, FLAVIN-ADENINE DINUCLEOTIDE, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: oxidoreductase
• 由来する生物種: Pseudomonas fluorescens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 64141.22

構造登録者

Shepherd, S.A.,Karthikeyan, C.,Latham, J.,Struck, A.-W.,Thompson, M.L.,Menon, B.,Levy, C.W.,Leys, D.,Micklefield, J. (登録日: 2015-04-01, 公開日: 2016-04-13, 最終更新日: 2024-01-10)

主引用文献

Shepherd, S.A.,Karthikeyan, C.,Latham, J.,Struck, A.W.,Thompson, M.L.,Menon, B.R.K.,Styles, M.Q.,Levy, C.,Leys, D.,Micklefield, J.
Extending the biocatalytic scope of regiocomplementary flavin-dependent halogenase enzymes.
Chem Sci, 6:3454-3460, 2015

PubMed Abstract: Flavin-dependent halogenases are potentially valuable biocatalysts for the regioselective halogenation of aromatic compounds. These enzymes, utilising benign inorganic halides, offer potential advantages over traditional non-enzymatic halogenation chemistry that often lacks regiocontrol and requires deleterious reagents. Here we extend the biocatalytic repertoire of the tryptophan halogenases, demonstrating how these enzymes can halogenate a range of alternative aryl substrates. Using structure guided mutagenesis we also show that it is possible to alter the regioselectivity as well as increase the activity of the halogenases with non-native substrates including anthranilic acid; an important intermediate in the synthesis and biosynthesis of pharmaceuticals and other valuable products.

PubMed: 29511510
DOI: 10.1039/c5sc00913h

実験手法

X-RAY DIFFRACTION (2.204 Å)