4Z3D

Human carbonyl reductase 1 with glutathione in a protective configuration

Summary for 4Z3D

• Entry DOI: 10.2210/pdb4z3d/pdb
• Descriptor: Carbonyl reductase [NADPH] 1, GLUTATHIONE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• Functional Keywords: glutathione, nadph, carbonyl reductase, oxidoreductase
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: P16152
• Total number of polymer chains: 4
• Total formula weight: 125349.99

Authors

Ding, Y.,Liang, Q. (deposition date: 2015-03-31, release date: 2015-10-14, Last modification date: 2024-03-20)

Primary citation

Liang, Q.,Liu, R.,Du, S.,Ding, Y.
Structural insights on the catalytic site protection of human carbonyl reductase 1 by glutathione.
J.Struct.Biol., 192:138-144, 2015

PubMed Abstract: The NADPH-dependent human carbonyl reductase 1 (hCBR1), a member of the short-chain dehydrogenase/reductase protein family, plays an important role in the ubiquitous metabolism of endogenous and xenobiotic carbonyl containing compounds. Glutathione (GSH) is also a cofactor of hCBR1, however, its role in the carbonyl reductase function of the enzyme is still unclear. In this study, we presented the crystal structure of hCBR1 in complex with GSH, in the absence of its substrates or inhibitors. Interestingly, we found that the GSH molecule presents in a configuration quite different from that was previously reported when substrate is binding to hCBR1. Our structure indicates that GSH contributes to the substrate selectivity of hCBR1 and protects the catalytic center of hCBR1 through a switch-like mechanism. The isothermal titration calorimetry and enzymology data shows that GSH directly binding with hCBR1 when there's no substrate exist. The enzymology data also shows GSH protects NADPH being attacked by oxidative small molecules. This is the first time that GSH is found to demonstrate such functions as a co-enzyme. Our crystal structure succeeds in providing critical insights into the substrate selectivity of hCBR1 and the interaction between hCBR1 and GSH.

PubMed: 26381805
DOI: 10.1016/j.jsb.2015.09.005

Experimental method

X-RAY DIFFRACTION (1.8 Å)