4Z1B

Structure of H204A mutant KDO8PS from H.pylori

Summary for 4Z1B

• Entry DOI: 10.2210/pdb4z1b/pdb
• Related: 4Z1A 4Z1C 4Z1D
• Descriptor: 2-dehydro-3-deoxyphosphooctonate aldolase (2 entities in total)
• Functional Keywords: helicobacter pylori, lipopolysaccharide biosynthesis, mutant, transferase
• Biological source: Helicobacter pylori (strain ATCC 700392 / 26695)
• Cellular location: Cytoplasm : P56060
• Total number of polymer chains: 2
• Total formula weight: 60629.70

Authors

Lee, B.J.,Cho, S.,Im, H.,Yoon, H.J. (deposition date: 2015-03-27, release date: 2016-03-09, Last modification date: 2024-03-20)

Primary citation

Cho, S.,Im, H.,Lee, K.Y.,Chen, J.,Kang, H.J.,Yoon, H.J.,Min, K.H.,Lee, K.R.,Park, H.J.,Lee, B.J.
Identification of novel scaffolds for potential anti-Helicobacter pylori agents based on the crystal structure of H. pylori 3-deoxy-d-manno-octulosonate 8-phosphate synthase (HpKDO8PS).
Eur.J.Med.Chem., 108:188-202, 2016

PubMed Abstract: The crystal structure of 3-deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from Helicobacter pylori (HpKDO8PS) was determined alone and within various complexes, revealing an extra helix (HE) that is absent in the structures of KDO8PS from other organisms. In contrast to the metal coordination of the KDO8PS enzyme from Aquifex aeolicus, HpKDO8PS is specifically coordinated with Cd(2+) or Zn(2+) ions, and isothermal titration calorimetry (ITC) and differential scanning fluorimetry (DSF) revealed that Cd(2+) thermally stabilizes the protein structure more efficiently than Zn(2+). In the substrate-bound structure, water molecules play a key role in fixing residues in the proper configuration to achieve a compact structure. Using the structures of HpKDO8PS and API [arabinose 5-phosphate (A5P) and phosphoenolpyruvate (PEP) bisubstrate inhibitor], we generated 21 compounds showing potential HpKDO8PS-binding properties via in silico virtual screening. The capacity of three, avicularin, hyperin, and MC181, to bind to HpKDO8PS was confirmed through saturation transfer difference (STD) experiments, and we identified their specific ligand binding modes by combining competition experiments and docking simulation analysis. Hyperin was confirmed to bind to the A5P binding site, primarily via hydrophilic interaction, whereas MC181 bound to both the PEP and A5P binding sites through hydrophilic and hydrophobic interactions. These results were consistent with the epitope mapping by STD. Our results are expected to provide clues for the development of HpKDO8PS inhibitors.

PubMed: 26649906
DOI: 10.1016/j.ejmech.2015.11.036

Experimental method

X-RAY DIFFRACTION (2.4 Å)