4Z1B
Structure of H204A mutant KDO8PS from H.pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.859, 50.869, 78.302 |
| Unit cell angles | 90.00, 104.74, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.18568 |
| Rwork | 0.183 |
| R-free | 0.23836 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.469 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 21294 |
| <I/σ(I)> | 38.45 |
| Completeness [%] | 96.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, HEPES, magnesium chloride |
