4YXC

Complex of FliM(SPOA)::FliN fusion protein and FliH(APAR)::T4lysozyme fusion protein

Summary for 4YXC

• Entry DOI: 10.2210/pdb4yxc/pdb
• Descriptor: Flagellar assembly protein H,Endolysin, Flagellar motor switch protein FliM,Flagellar motor switch protein FliN (3 entities in total)
• Functional Keywords: type iii secretion system, protein transport
• Biological source: Salmonella enterica subsp. enterica serovar Enteritidis str. SE30663; More
• Cellular location: Host cytoplasm : P00720; Cell inner membrane; Peripheral membrane protein; Cytoplasmic side: P26419
• Total number of polymer chains: 2
• Total formula weight: 45698.98

Authors

Notti, R.Q.,Stebbins, C.E. (deposition date: 2015-03-23, release date: 2015-06-03, Last modification date: 2023-09-27)

Primary citation

Notti, R.Q.,Bhattacharya, S.,Lilic, M.,Stebbins, C.E.
A common assembly module in injectisome and flagellar type III secretion sorting platforms.
Nat Commun, 6:7125-7125, 2015

PubMed Abstract: Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarchical secretion of protein substrates, its assembly and regulation remain unclear. Here we describe a module mediating the assembly of the sorting platform in both secretion systems, and elucidate the structural basis for segregation of homologous components among these divergent T3SS subtypes sharing a common cytoplasmic milieu. These results provide a foundation for the subtype-specific assembly of T3SS sorting platforms and will support further mechanistic analysis and anti-virulence drug design.

PubMed: 25994170
DOI: 10.1038/ncomms8125

Experimental method

X-RAY DIFFRACTION (2.3 Å)