4YK8

Crystal structure of the Atg101-Atg13 complex from fission yeast

Summary for 4YK8

• Entry DOI: 10.2210/pdb4yk8/pdb
• Descriptor: Meiotically up-regulated gene 66 protein, Autophagy protein 13 (2 entities in total)
• Functional Keywords: autophagy, horma, protein transport
• Biological source: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); More
• Total number of polymer chains: 2
• Total formula weight: 47880.88

Authors

Suzuki, H.,Noda, N.N. (deposition date: 2015-03-04, release date: 2015-06-03, Last modification date: 2024-03-20)

Primary citation

Suzuki, H.,Kaizuka, T.,Mizushima, N.,Noda, N.N.
Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation.
Nat.Struct.Mol.Biol., 22:572-580, 2015

PubMed Abstract: Atg101 is an essential component of the autophagy-initiating ULK complex in higher eukaryotes, but it is absent from the functionally equivalent Atg1 complex in budding yeast. Here, we report the crystal structure of the fission yeast Atg101-Atg13 complex. Atg101 has a Hop1, Rev7 and Mad2 (HORMA) architecture similar to that of Atg13. Mad2 HORMA has two distinct conformations (O-Mad2 and C-Mad2), and, intriguingly, Atg101 resembles O-Mad2 rather than the C-Mad2-like Atg13. Atg13 HORMA from higher eukaryotes possesses an inherently unstable fold, which is stabilized by Atg101 via interactions analogous to those between O-Mad2 and C-Mad2. Mutational studies revealed that Atg101 is responsible for recruiting downstream factors to the autophagosome-formation site in mammals via a newly identified WF finger. These data define the molecular functions of Atg101, providing a basis for elucidating the molecular mechanisms of mammalian autophagy initiation by the ULK complex.

PubMed: 26030876
DOI: 10.1038/nsmb.3036

Experimental method

X-RAY DIFFRACTION (3 Å)