4Y4P

Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution

これはPDB形式変換不可エントリーです。

4Y4P の概要

• エントリーDOI: 10.2210/pdb4y4p/pdb
• 関連するPDBエントリー: 4Y4O
• 分子名称: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (60 entities in total)
• 機能のキーワード: ribosome, rrna modofications, pseudouridine, rrna methylation, ribosomal protein post-translational modifications, iron-sulfur cluster, beta-thio-aspartate
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 112
• 化学式量合計: 4571395.35

構造登録者

Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A. (登録日: 2015-02-10, 公開日: 2015-03-18, 最終更新日: 2025-03-19)

主引用文献

Polikanov, Y.S.,Melnikov, S.V.,Soll, D.,Steitz, T.A.
Structural insights into the role of rRNA modifications in protein synthesis and ribosome assembly.
Nat.Struct.Mol.Biol., 22:342-344, 2015

PubMed Abstract: We report crystal structures of the Thermus thermophilus ribosome at 2.3- to 2.5-Å resolution, which have enabled modeling of rRNA modifications. The structures reveal contacts of modified nucleotides with mRNA and tRNAs or protein pY, and contacts within the ribosome interior stabilizing the functional fold of rRNA. Our work provides a resource to explore the roles of rRNA modifications and yields a more comprehensive atomic model of a bacterial ribosome.

PubMed: 25775268
DOI: 10.1038/nsmb.2992

実験手法

X-RAY DIFFRACTION (2.5 Å)