4Y0P

Bovine beta-lactoglobulin complex with tetracaine (BLG-TET)

Summary for 4Y0P

• Entry DOI: 10.2210/pdb4y0p/pdb
• Descriptor: Beta-lactoglobulin, Tetracaine (3 entities in total)
• Functional Keywords: transport, ligand binding
• Biological source: Bos taurus (cattle)
• Cellular location: Secreted: P02754
• Total number of polymer chains: 1
• Total formula weight: 18565.54

Authors

Loch, J.I.,Bonarek, P.,Polit, A.,Jablonski, M.,Czub, M.,Ye, X.,Lewinski, K. (deposition date: 2015-02-06, release date: 2015-07-01, Last modification date: 2024-10-23)

Primary citation

Loch, J.I.,Bonarek, P.,Polit, A.,Jabonski, M.,Czub, M.,Ye, X.,Lewinski, K.
beta-Lactoglobulin interactions with local anaesthetic drugs - Crystallographic and calorimetric studies.
Int.J.Biol.Macromol., 80:87-94, 2015

PubMed Abstract: Interactions between bovine and goat β-lactoglobulin and tetracaine and pramocaine were investigated with isothermal titration calorimetry, X-ray crystallography and molecular modelling. Tetracaine and pramocaine binding to lactoglobulin is an entropy driven endothermic reaction. In this work, we found that determined association constants and thermodynamic parameters indicate that pramocaine has a higher affinity to lactoglobulin than tetracaine. Crystal structures that were determined with resolutions in the range from 1.90 to 2.30 Å revealed in each case the presence of a single drug molecule bound in the β-barrel in a mode similar to that observed for 14- and 16-carbon fatty acids. The position of the ligand in the β-barrel indicates the optimal fit of 6-carbon aromatic rings to the binding pocket and the major role of hydrophobic interactions in ligand binding. Calculations of tetracaine and pramocaine docking to lactoglobulin revealed that molecular modelling overestimated the role of polar protein-drug interactions.

PubMed: 26092174
DOI: 10.1016/j.ijbiomac.2015.06.013

Experimental method

X-RAY DIFFRACTION (2.2 Å)