4XYI

Mis16 with H4 peptide

Summary for 4XYI

• Entry DOI: 10.2210/pdb4xyi/pdb
• Related: 4XYH
• Descriptor: Kinetochore protein Mis16, Histone H4 (2 entities in total)
• Functional Keywords: centromere, cenp-a, kinetochore, mis18 complex, histone, chaperone
• Biological source: Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast); More
• Total number of polymer chains: 2
• Total formula weight: 59882.98

Authors

An, S.,Kim, H.,Cho, U.-S. (deposition date: 2015-02-02, release date: 2016-02-03, Last modification date: 2023-09-27)

Primary citation

An, S.,Kim, H.,Cho, U.S.
Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.
J.Mol.Biol., 427:3230-3240, 2015

PubMed Abstract: CENP-A is a centromere-specific histone H3 variant that is required for kinetochore assembly and accurate chromosome segregation. For it to function properly, CENP-A must be specifically localized to centromeres. In fission yeast, Scm3sp and the Mis18 complex, composed of Mis16, Eic1, and Mis18, function as a CENP-A(Cnp1)-specific chaperone and a recruiting factor, respectively, and together ensure accurate delivery of CENP-A(Cnp1) to centromeres. Although how Scm3sp specifically recognizes CENP-A(Cnp1) has been revealed recently, the recruiting mechanism of CENP-A(Cnp1) via the Mis18 complex remains unknown. In this study, we have determined crystal structures of Schizosaccharomyces japonicus Mis16 alone and in complex with the helix 1 of histone H4 (H4α1). Crystal structures followed by mutant analysis and affinity pull-downs have revealed that Mis16 recognizes both H4α1 and Scm3sp independently within the CENP-A(Cnp1)/H4:Scm3sp complex. This observation suggests that Mis16 gains CENP-A(Cnp1) specificity by recognizing both Scm3sp and histone H4. Our studies provide insights into the molecular mechanisms underlying specific recruitment of CENP-A(Cnp1)/H4:Scm3sp into centromeres.

PubMed: 26343758
DOI: 10.1016/j.jmb.2015.08.022

Experimental method

X-RAY DIFFRACTION (3 Å)