4XYH

Wild-type full length Mis16 in Schizosaccharomyces japonicus

4XYH の概要

• エントリーDOI: 10.2210/pdb4xyh/pdb
• 関連するPDBエントリー: 4XYI
• 分子名称: Kinetochore protein Mis16 (2 entities in total)
• 機能のキーワード: centromere, cenp-a, kinetochore, mis18 complex, histone, chaperone
• 由来する生物種: Schizosaccharomyces japonicus (strain yFS275 / FY16936) (Fission yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48462.54

構造登録者

An, S.,Kim, H.,Cho, U.-S. (登録日: 2015-02-02, 公開日: 2016-01-13, 最終更新日: 2023-09-27)

主引用文献

An, S.,Kim, H.,Cho, U.S.
Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.
J.Mol.Biol., 427:3230-3240, 2015

PubMed Abstract: CENP-A is a centromere-specific histone H3 variant that is required for kinetochore assembly and accurate chromosome segregation. For it to function properly, CENP-A must be specifically localized to centromeres. In fission yeast, Scm3sp and the Mis18 complex, composed of Mis16, Eic1, and Mis18, function as a CENP-A(Cnp1)-specific chaperone and a recruiting factor, respectively, and together ensure accurate delivery of CENP-A(Cnp1) to centromeres. Although how Scm3sp specifically recognizes CENP-A(Cnp1) has been revealed recently, the recruiting mechanism of CENP-A(Cnp1) via the Mis18 complex remains unknown. In this study, we have determined crystal structures of Schizosaccharomyces japonicus Mis16 alone and in complex with the helix 1 of histone H4 (H4α1). Crystal structures followed by mutant analysis and affinity pull-downs have revealed that Mis16 recognizes both H4α1 and Scm3sp independently within the CENP-A(Cnp1)/H4:Scm3sp complex. This observation suggests that Mis16 gains CENP-A(Cnp1) specificity by recognizing both Scm3sp and histone H4. Our studies provide insights into the molecular mechanisms underlying specific recruitment of CENP-A(Cnp1)/H4:Scm3sp into centromeres.

PubMed: 26343758
DOI: 10.1016/j.jmb.2015.08.022

実験手法

X-RAY DIFFRACTION (2.3 Å)