4XYH

Wild-type full length Mis16 in Schizosaccharomyces japonicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	105
Detector technology	CCD
Collection date	2014-02-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 65
Unit cell lengths	134.342, 134.342, 72.133
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.158 - 2.300
R-factor	0.1765
Rwork	0.174
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cfs
RMSD bond length	0.008
RMSD bond angle	1.134
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.158	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	30905
<I/σ(I)>	34.1	3.2
Completeness [%]	93.4	96.9
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M calcium acetate 0.1 M sodium cacodylate 18% PEG 8000