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4XRR

Crystal structure of cals8 from micromonospora echinospora (P294S mutant)

Replaces:  4WB1
Summary for 4XRR
Entry DOI10.2210/pdb4xrr/pdb
DescriptorCalS8, GLYCEROL (3 entities in total)
Functional Keywordscalicheamicin, oxidoreductase, structural genomics, psi-biology, midwest center for structural genomics, mcsg, enzyme discovery for natural product biosynthesis, natpro
Biological sourceMicromonospora echinospora
Total number of polymer chains2
Total formula weight96175.68
Authors
Primary citationSingh, S.,Michalska, K.,Bigelow, L.,Endres, M.,Kharel, M.K.,Babnigg, G.,Yennamalli, R.M.,Bingman, C.A.,Joachimiak, A.,Thorson, J.S.,Phillips, G.N.
Structural Characterization of CalS8, a TDP-alpha-D-Glucose Dehydrogenase Involved in Calicheamicin Aminodideoxypentose Biosynthesis.
J. Biol. Chem., 290:26249-26258, 2015
Cited by
PubMed Abstract: Classical UDP-glucose 6-dehydrogenases (UGDHs; EC 1.1.1.22) catalyze the conversion of UDP-α-d-glucose (UDP-Glc) to the key metabolic precursor UDP-α-d-glucuronic acid (UDP-GlcA) and display specificity for UDP-Glc. The fundamental biochemical and structural study of the UGDH homolog CalS8 encoded by the calicheamicin biosynthetic gene is reported and represents one of the first studies of a UGDH homolog involved in secondary metabolism. The corresponding biochemical characterization of CalS8 reveals CalS8 as one of the first characterized base-permissive UGDH homologs with a >15-fold preference for TDP-Glc over UDP-Glc. The corresponding structure elucidations of apo-CalS8 and the CalS8·substrate·cofactor ternary complex (at 2.47 and 1.95 Å resolution, respectively) highlight a notably high degree of conservation between CalS8 and classical UGDHs where structural divergence within the intersubunit loop structure likely contributes to the CalS8 base permissivity. As such, this study begins to provide a putative blueprint for base specificity among sugar nucleotide-dependent dehydrogenases and, in conjunction with prior studies on the base specificity of the calicheamicin aminopentosyltransferase CalG4, provides growing support for the calicheamicin aminopentose pathway as a TDP-sugar-dependent process.
PubMed: 26240141
DOI: 10.1074/jbc.M115.673459
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.55 Å)
Structure validation

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건을2025-07-23부터공개중

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