4XJR

The catalytic mechanism of human parainfluenza virus type 3 haemagglutinin-neuraminidase revealed

Summary for 4XJR

• Entry DOI: 10.2210/pdb4xjr/pdb
• Related: 4XJQ
• Descriptor: Hemagglutinin-neuraminidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: hydrolase, human parainfluenza virus 3, haemagglutinin-neuraminidase, sialidase mechanism
• Biological source: Human parainfluenza virus 3
• Total number of polymer chains: 2
• Total formula weight: 104225.56

Authors

Dirr, L.,El-Deeb, I.,Guillon, P.,Carroux, C.,Chavas, L.,von Itzstein, M. (deposition date: 2015-01-08, release date: 2015-02-11, Last modification date: 2024-11-06)

Primary citation

Dirr, L.,El-Deeb, I.M.,Guillon, P.,Carroux, C.J.,Chavas, L.M.,von Itzstein, M.
The catalytic mechanism of human parainfluenza virus type 3 haemagglutinin-neuraminidase revealed.
Angew.Chem.Int.Ed.Engl., 54:2936-2940, 2015

PubMed Abstract: Human parainfluenza virus type 3 (hPIV-3) is one of the leading causes for lower respiratory tract disease in children, with neither an approved antiviral drug nor vaccine available to date. Understanding the catalytic mechanism of human parainfluenza virus haemagglutinin-neuraminidase (HN) protein is key to the design of specific inhibitors against this virus. Herein, we used (1) H NMR spectroscopy, X-ray crystallography, and virological assays to study the catalytic mechanism of the HN enzyme activity and have identified the conserved Tyr530 as a key amino acid involved in catalysis. A novel 2,3-difluorosialic acid derivative showed prolonged enzyme inhibition and was found to react and form a covalent bond with Tyr530. Furthermore, the novel derivative exhibited enhanced potency in virus blockade assays relative to its Neu2en analogue. These outcomes open the door for a new generation of potent inhibitors against hPIV-3 HN.

PubMed: 25676091
DOI: 10.1002/anie.201412243

Experimental method

X-RAY DIFFRACTION (3 Å)