4XFS

Structure of IL-18 SER Mutant I

4XFS の概要

• エントリーDOI: 10.2210/pdb4xfs/pdb
• 分子名称: Interleukin-18, DIMETHYL SULFOXIDE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: interleukin-18, il-18, cytokine, immune defense, surface entropy reduction
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36505.60

構造登録者

Krumm, B.E.,Meng, X.,Xiang, Y.,Deng, J. (登録日: 2014-12-28, 公開日: 2015-06-10, 最終更新日: 2023-09-27)

主引用文献

Krumm, B.,Meng, X.,Xiang, Y.,Deng, J.
Crystallization of interleukin-18 for structure-based inhibitor design.
Acta Crystallogr.,Sect.F, 71:710-717, 2015

PubMed Abstract: Interleukin-18 (IL-18) is a pleiotropic pro-inflammatory cytokine belonging to the IL-1 superfamily. IL-18 plays an important role in host innate and acquired immune defense, with its activity being modulated in vivo by its naturally occurring antagonist IL-18 binding protein (IL-18BP). Recent crystal structures of human IL-18 (hIL-18) in complex with its antagonist or cognate receptor(s) have revealed a conserved binding interface on hIL-18 representing a promising drug target. An important step in this process is obtaining crystals of apo hIL-18 or hIL-18 in complex with small-molecule inhibitors, preferably under low ionic strength conditions. In this study, surface-entropy reduction (SER) and rational protein design were employed to facilitate the crystallization of hIL-18. The results provide an excellent platform for structure-based drug design.

PubMed: 26057800
DOI: 10.1107/S2053230X15006871

実験手法

X-RAY DIFFRACTION (1.91 Å)