4X9J

EGR-1 with Doubly Methylated DNA

4X9J の概要

• エントリーDOI: 10.2210/pdb4x9j/pdb
• 関連するPDBエントリー: 4R2A
• 分子名称: Early growth response protein 1, DNA (5'-D(*AP*GP*(5CM)P*GP*TP*GP*GP*GP*(5CM)P*GP*T)-3'), DNA (5'-D(*TP*AP*(5CM)P*GP*CP*CP*CP*AP*(5CM)P*GP*C)-3'), ... (5 entities in total)
• 機能のキーワード: dna binding, methylated dna, zinc finger, transcription, transcription regulator-dna complex, transcription regulator/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : P18146
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 17684.02

構造登録者

White, M.A.,Zandarashvili, L.,Iwahara, J. (登録日: 2014-12-11, 公開日: 2015-05-13, 最終更新日: 2023-09-27)

主引用文献

Zandarashvili, L.,White, M.A.,Esadze, A.,Iwahara, J.
Structural impact of complete CpG methylation within target DNA on specific complex formation of the inducible transcription factor Egr-1.
Febs Lett., 589:1748-1753, 2015

PubMed Abstract: The inducible transcription factor Egr-1 binds specifically to 9-bp target sequences containing two CpG sites that can potentially be methylated at four cytosine bases. Although it appears that complete CpG methylation would make an unfavorable steric clash in the previous crystal structures of the complexes with unmethylated or partially methylated DNA, our affinity data suggest that DNA recognition by Egr-1 is insensitive to CpG methylation. We have determined, at a 1.4-Å resolution, the crystal structure of the Egr-1 zinc-finger complex with completely methylated target DNA. Structural comparison of the three different methylation states reveals why Egr-1 can recognize the target sequences regardless of CpG methylation.

PubMed: 25999311
DOI: 10.1016/j.febslet.2015.05.022

実験手法

X-RAY DIFFRACTION (1.412 Å)