4X98

Immunoglobulin Fc heterodimer variant

4X98 の概要

• エントリーDOI: 10.2210/pdb4x98/pdb
• 関連するPDBエントリー: 4X99
• 分子名称: Ig gamma-1 chain C region, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: bispecific antibody, immunoglobulin fc heterodimer, ch3 domain interface, asymmetric disulfide bonds, thermal stability, fc engineering, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Secreted: P01857 P01857
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51342.41

構造登録者

Seok, S.H.,Choi, H.J.,Kim, Y.J.,Seo, M.D.,Kim, Y.S. (登録日: 2014-12-11, 公開日: 2015-06-03, 最終更新日: 2024-10-16)

主引用文献

Choi, H.J.,Seok, S.H.,Kim, Y.J.,Seo, M.D.,Kim, Y.S.
Crystal structures of immunoglobulin Fc heterodimers reveal the molecular basis for heterodimer formation.
Mol.Immunol., 65:377-383, 2015

PubMed Abstract: We determined the X-ray crystal structure of an immunoglobulin fragment crystallizable (Fc) heterodimer, EW-RVT, at a resolution of 2.5Å and found that the designed asymmetric interaction residues located in the heterodimeric CH3 interface favor Fc heterodimer formation. We further generated an inter-CH3 disulfide-bonded heterodimeric Fc variant, EW-RVT(S-S), which exhibited improved heterodimer formation and thermodynamic stability compared with the parent EW-RVT variant. The crystal structure of EW-RVTS-S superimposed very closely with the wild-type Fc structure. Our results provide the detailed structure of heterodimeric Fc scaffolds, which will be useful for the generation of immunoglobulin G (IgG)-like bispecific antibodies.

PubMed: 25743157
DOI: 10.1016/j.molimm.2015.02.017

実験手法

X-RAY DIFFRACTION (2.499 Å)