4X55

Structure of the class D Beta-Lactamase OXA-225 K82D in Acyl-Enzyme Complex with Ceftazidime

4X55 の概要

• エントリーDOI: 10.2210/pdb4x55/pdb
• 関連するPDBエントリー: 4X53 4X56
• 分子名称: Beta-lactamase OXA-225, 1-({(2R)-2-[(1R)-1-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}-2-oxoethyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-5-yl}methyl)pyridinium (3 entities in total)
• 機能のキーワード: hydrolase, carbapenemase, antibiotic
• 由来する生物種: Acinetobacter baumannii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58621.34

構造登録者

Clasman, J.R.,June, C.M.,Powers, R.A.,Leonard, D.A. (登録日: 2014-12-04, 公開日: 2015-03-11, 最終更新日: 2024-10-23)

主引用文献

Mitchell, J.M.,Clasman, J.R.,June, C.M.,Kaitany, K.C.,LaFleur, J.R.,Taracila, M.A.,Klinger, N.V.,Bonomo, R.A.,Wymore, T.,Szarecka, A.,Powers, R.A.,Leonard, D.A.
Structural Basis of Activity against Aztreonam and Extended Spectrum Cephalosporins for Two Carbapenem-Hydrolyzing Class D beta-Lactamases from Acinetobacter baumannii.
Biochemistry, 54:1976-1987, 2015

PubMed Abstract: The carbapenem-hydrolyzing class D β-lactamases OXA-23 and OXA-24/40 have emerged worldwide as causative agents for β-lactam antibiotic resistance in Acinetobacter species. Many variants of these enzymes have appeared clinically, including OXA-160 and OXA-225, which both contain a P → S substitution at homologous positions in the OXA-24/40 and OXA-23 backgrounds, respectively. We purified OXA-160 and OXA-225 and used steady-state kinetic analysis to compare the substrate profiles of these variants to their parental enzymes, OXA-24/40 and OXA-23. OXA-160 and OXA-225 possess greatly enhanced hydrolytic activities against aztreonam, ceftazidime, cefotaxime, and ceftriaxone when compared to OXA-24/40 and OXA-23. These enhanced activities are the result of much lower Km values, suggesting that the P → S substitution enhances the binding affinity of these drugs. We have determined the structures of the acylated forms of OXA-160 (with ceftazidime and aztreonam) and OXA-225 (ceftazidime). These structures show that the R1 oxyimino side-chain of these drugs occupies a space near the β5-β6 loop and the omega loop of the enzymes. The P → S substitution found in OXA-160 and OXA-225 results in a deviation of the β5-β6 loop, relieving the steric clash with the R1 side-chain carboxypropyl group of aztreonam and ceftazidime. These results reveal worrying trends in the enhancement of substrate spectrum of class D β-lactamases but may also provide a map for β-lactam improvement.

PubMed: 25710192
DOI: 10.1021/bi501547k

実験手法

X-RAY DIFFRACTION (1.941 Å)