4X0U

Structure ALDH7A1 inactivated by 4-diethylaminobenzaldehyde

4X0U の概要

• エントリーDOI: 10.2210/pdb4x0u/pdb
• 関連するPDBエントリー: 4X0T
• 分子名称: Alpha-aminoadipic semialdehyde dehydrogenase, 4-(diethylamino)benzaldehyde, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: aldehyde dehydrogenase, nad, oxidoreductase, lysine catabolism, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm, cytosol . Isoform 1: Mitochondrion : P49419
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 223214.74

構造登録者

Luo, M.,Tanner, J.J. (登録日: 2014-11-23, 公開日: 2015-01-14, 最終更新日: 2024-10-16)

主引用文献

Luo, M.,Gates, K.S.,Henzl, M.T.,Tanner, J.J.
Diethylaminobenzaldehyde Is a Covalent, Irreversible Inactivator of ALDH7A1.
Acs Chem.Biol., 10:693-697, 2015

PubMed Abstract: There is growing interest in aldehyde dehydrogenases (ALDHs) because of their overexpression in cancer stem cells and the ability to mediate resistance to cancer drugs. Here, we report the first crystal structure of an aldehyde dehydrogenase complexed with the inhibitor 4-diethylaminobenzaldehyde (DEAB). Contrary to the widely held belief that DEAB is a reversible inhibitor of ALDHs, we show that DEAB irreversibly inactivates ALDH7A1 via formation of a stable, covalent acyl-enzyme species.

PubMed: 25554827
DOI: 10.1021/cb500977q

実験手法

X-RAY DIFFRACTION (1.95 Å)